SELECTIVE REOVIRUS INFECTION OF MURINE HEPATOCARCINOMA CELLS DURING CELL-DIVISION - A MODEL OF VIRAL LIVER INFECTION

Citation
J. Taterka et al., SELECTIVE REOVIRUS INFECTION OF MURINE HEPATOCARCINOMA CELLS DURING CELL-DIVISION - A MODEL OF VIRAL LIVER INFECTION, The Journal of clinical investigation, 94(1), 1994, pp. 353-360
Citations number
40
Categorie Soggetti
Medicine, Research & Experimental
ISSN journal
00219738
Volume
94
Issue
1
Year of publication
1994
Pages
353 - 360
Database
ISI
SICI code
0021-9738(1994)94:1<353:SRIOMH>2.0.ZU;2-5
Abstract
Reovirus type 1, strain Lang (1/L), can infect hepatocytes in vivo onl y after hepatocellular damage is induced by hepatotoxins, surgical tra uma, resection, or profound immunosuppression. To examine the role of cell cycle and cellular differentiation on liver cell susceptibility t o reovirus infection, a murine hepatocarcinoma cell line, Hepa 1/A1, w as infected with reovirus and assayed for the presence of infectious v irus or reovirus antigen in cells. Despite a > 95% binding of reovirus to hepatocarcinoma cells as indicated by cytometric analysis; only 10 % of hepatoma cells contained infectious virus by infectious center as say. In comparison, 100% of L cells were infected. Analysis of intrace llular reovirus antigen revealed its presence in dividing but not in q uiescent hepatocytes. This correlation of cellular division and cell c apacity to support viral replication suggests that induction of hepato cyte proliferation may be a mechanism for liver susceptibility to reov irus infection.