Repeated subculturing of Rhodobacter capsulatus strain BK5 under photo
trophic conditions on a medium containing butyrate and nitrate led to
the appearance of cultures that, unlike the original, produced gas. Is
olation of a pure culture of the gas-forming organism revealed that it
was a derivative of R. capsulatus BK5 that by virtue of expressing a
nitrite reductase can catalyse the complete sequence of the denitrific
ation reactions. The nitrite reductase is of the type that contains co
pper rather than haem.