EFFECTS OF IODIZED OIL ON TRIVALENT ORAL POLIO VACCINE IN-VITRO

Citation
Jh. Bruning et al., EFFECTS OF IODIZED OIL ON TRIVALENT ORAL POLIO VACCINE IN-VITRO, International journal for vitamin and nutrition research, 64(2), 1994, pp. 125-129
Citations number
6
Categorie Soggetti
Nutrition & Dietetics
ISSN journal
03009831
Volume
64
Issue
2
Year of publication
1994
Pages
125 - 129
Database
ISI
SICI code
0300-9831(1994)64:2<125:EOIOOT>2.0.ZU;2-9
Abstract
Iodine deficiency can be effectively reversed by administration of iod ized oil (IO). Since 1989, the WHO Expanded Programme on Immunization recommended delivery of oral IO along with vaccines, in appropriate si tuations. However, administration of IO at the same time as trivalent oral polio vaccine (TOPV) has not been recommended because of absence of studies addressing potential harmful effects of iodized oil on immu no-responses to TOPV. In this study an in-vitro cell culture system wa s used to measure possible adverse effects of IO on TOPV. The system i tself was shown not to be influenced by either IO or a standard non-io dized oil (NIO). However, marked effects of IO on virus infectivity wa s measured when a 50% mixture of IO and TOPV was incubated for 48 h at 37-degrees-C, as compared to incubation without IO. Clear differences were found in effects of IO on vaccines of different sources, suggest ing differences in composition of these vaccines, e.g. in stabilisers. Principally Sabin 3 appeared susceptible to IO under the given extrem e conditions. Pre-dilution of the IO-TOPV mixture prior to incubation clearly reduced the effects of IO on virus infectivity Inoculation of the IO/TOPV-mixture directly onto susceptible cells, a model approachi ng the in vivo situation, resulted in no detrimental effects on virus infectivity. Further studies are planned looking at interactions of io dized oil and TOPV in children at immunization contacts.