A sterol-polyether conjugate, 5-androstene-3 beta,17 beta-bis[(oxycarb
onyl)hexaethylene glycol] (1), has been synthesized via condensation o
f the bis-chloroformate derivative of 5-androstene-3 beta,17 beta-diol
with oxyethylene glycol mono(triphenylmethyl ether), followed by depr
otection. This conjugate, which is composed of a long and rigid hydrop
hobic unit, two flexible hydrophilic chains that are linked to the hyd
rophobic unit, and a pendant polar head group, was designed as a proto
type for new classes of compounds that are intended to serve as functi
onal equivalents of the polyene macrolide antibiotic amphotericin B. A
nalysis of the surface pressure-area isotherm of 1, generated at the a
ir-water interface, indicates a limiting area of ca. 60 Angstrom(2) pe
r molecule. This value is fully consistent with a model in which the s
terol nucleus (ca. 40 Angstrom(2)) and one pendant polyether chain (ca
. 20 Angstrom(2)) define the collisional area of the surfactant; that
is, it supports the existence of a ''folded'' conformation at the air-
water interface. Incorporation of 1 into egg PC vesicular membranes le
ads to ion channel formation, as demonstrated by Na-23 NMR spectroscop
y. Operationally, 1 has been found to have an ionophoric activity that
-is very similar to that found for a synthetic ''bouquet'' molecule, b
ut significantly less than amphotericin B, itself.