DESCRIPTION OF GENETIC DIVERSITY WITHIN B RASSICA-OLERACEA USING ISOENZYMATIC AND MOLECULAR MARKERS

The aim of this work is to construct homogeneous genetic pools for the multiplication of a crop collection of Brassica oleracea. Two methods of analysis of genetic diversity were compared. For intra-population investigations, the isoenzymatic method is more efficient. However, fo r studying population structure, the information given by RAPD (random ly amplified DNA sequence) is better correlated with morphological and agronomical diversity and is less expensive. The suggested method inv olves testing for the presence or absence of about 100,RAPD markers in a sample of mixed DNA from 40 seeds. The appropriate structure for th e collection can be determined from inter-population and inter-cultigr oup distances estimated by this technique. The data allow the construc tion of phylogenetic trees by the PA UP software (phylogenetic analysi s using parsimony).