New data on mitochondrial DNA polymorphism in Oryctolagus cuniculus co
nfirm the existence of 2 maternal lineages which are geographically we
ll separated. They provide evidence in favour of northern Spain (and p
ossibly southern France) as a refuge area for rabbit populations durin
g the last major glaciation. Osteological analysis leads to the discri
mination of populations and the recognition of discrete qualitative ch
aracters, which provide additional markers to describe population dive
rsity. Characterization of different domains of mtDNA from ancient bon
es was used as a tool to resolve the general question of the origin of
present populations. Results obtained from ancient and present rabbit
s living in Zembra (Tunisia) showed that the present-day population ha
s descended from animals present on the island some 2 000 years ago. A
rchaeozoological data provide evidence for their introduction by Bronz
e Age, Punic or Roman people.