CHLORAMPHENICOL EXTRACTION FROM MILK BY USING THE DIPHASIC DIALYSIS METHOD FOLLOWED BY LIQUID-CHROMATOGRAPHIC DETERMINATION

An economical, fast, and simple method for the extraction and liquid c hromatographic determination of chloramphenicol (CAP) in milk is descr ibed. CAP is extracted by using a recently developed membrane-based me thod named ''diphasic dialysis.'' CAP is detected and quantitated in t he organic solvent used in dialysis without additional cleanup steps b y reversed-phase liquid chromatography and UV detection (270 nm). The determination limit of CAP in milk was about 5 mug/L, although as litt le as 1 mug/L could be detected under optimal working conditions.