LIQUID-CHROMATOGRAPHIC DETERMINATION OF FURAZOLIDONE IN SHRIMP

A liquid chromatographic (LC) method was developed for the quantitatio n of furazolidone residues in shrimp muscle. The shrimp homogenate (1. 0 g) is extracted with acetonitrile, and the extract is taken to dryne ss. The residue is dissolved in acetonitrile, and the solution is pass ed through alumina and C18 cleanup columns. The eluate is taken to dry ness and reconstituted in a suitable solvent for reversed-phase (C18) LC with UV detection at 365 nm. Recoveries of furazolidone from shrimp homogenates spiked from 5 to 80 ng/g ranged from 74.3 to 79.7%, and r elative standard deviations (RSDs) were 5.0-8.9%. RSDs for incurred fu razolidone quantitated at 5.9 and 9.2 ng/g were 6.6 and 7.6%, respecti vely.