ANALYSIS OF WORTMANNIN (H-1) BY LIQUID-CHROMATOGRAPHY AND GAS-CHROMATOGRAPHY

An analytical method for detection of wortmannin in corn kernels was d eveloped based on liquid chromatography (LC) and gas chromatography (G C). The sample was extracted with ethyl acetate, subjected to cleanup procedures on Sep-Pak silica gel column, and eluted with dichlorometha ne-chloroform (1 + 1). Analysis was made either by LC or GC. The limit of detection was 2.5 by LC and 25 ng/g by GC. The recovery from corn by LC at 2.5, 5.0, 10, 25, 50, 100, 250, 500, and 1000 ng/g was 89 (11 .5 sd), 72 (2.6), 69 (7.4), 90 (7.5), 78 (9.3), 72 (6.1), 84 (17.5), 8 2 (11.5), and 76 (12.9), respectively. Recovery measured by GC at 25, 50, 100, 250, 500, and 1000 ng/g was 78 (12.5), 72 (14.0), 78 (3.8), 6 9 (2.6), 74 (16.9) and 81 (8.3)%, respectively. The average recovery w as 79% by LC and 75% by GC.