M. Iglesias et Jpg. Ballesta, MECHANISM OF RESISTANCE TO THE ANTIBIOTIC TRICHOTHECIN IN THE PRODUCING FUNGI, European journal of biochemistry, 223(2), 1994, pp. 447-453
Trichothecium roseum, an imperfecti fungus producer of the translation
inhibitor trichothecin, is constitutively resistant to its product. F
usarium oxysporum, a fungi not described as a toxin producer, is sensi
tive to trichothecin but becomes resistant when grown in the presence
of the drug. In both cases, the resistance occurs at the level of the
ribosomes. In cell-free polypeptide polymerization systems, trichothec
in resistance is associated with the presence of 60S subunits from the
resistant organisms. Resistant ribosomes can be prepared in vitro by
incubating sensitive ribosomes, from either non-induced F. oxysporum o
r Saccharomyces cerevisiae,with cell extracts from the resistant cells
in the presence of S-adenosylmethionine. An in-vitro specific differe
ntial methylation is detected in the sensitive ribosomes but not in re
sistant particles using radioactive S-adenosylmethionine. The results
indicate for the first time the existence in eukaryotic organims of an
antibiotic-resistance mechanism involving a ribosomal methylation sim
ilar to that described previously in prokaryotic systems.