IN-VITRO REGENERATION OF FEMALE PLANTS OF SIMMONDSIA-CHINENSIS (LINK)SCHNEIDER (JOJOBA) USING COPPICE SHOOTS

Jojoba-plants are trained during cultivation in our country and the nu merous coppice shoots produced during subsequent years are removed per iodically. Nodal segments obtained from these coppice shoots of 10 yea r-old female plants were successfully used for in vitro regeneration. Nodal segments produced axillary shoots on Modified Murashige and Skoo g medium (MS-1) within a month. These shoots were incubated in dark in a liquid medium (MS-2) for 72 h (for early root initiation on subsequ ent medium) and then transferred to rooting medium (MS-3) where root i nitiation could be observed within a week. More than 80% shoots rooted in a months period.