A new optical-lever based atomic force microscope is described in whic
h the cantilever scans and is accurately tracked by a scanning focused
spot. It can operate at forces below one nanoNewton over image areas
greater than 100 mu X 100 mu. It can be combined with optical microsco
pes of high numerical aperture and operated with the sample and cantil
ever in fluids. As examples of its applications, images of living cell
s in Petri dishes and a 6 in. (15.24 mm) silicon wafer are included.