PREDICTING PROTEIN CRYSTALLIZATION FROM A DILUTE-SOLUTION PROPERTY

A dilute solution parameter obtained from static light-scattering meas urements is proposed as a predictor for protein crystallization experi ments. The osmotic second virial coefficients, B22, have been measured for a variety of proteins in solvents that are known to promote cryst allization and the values for B22 were found to lie within a fairly na rrow range which we refer to as a crystallization slot. Solution condi tions which were known not to favor crystallization of the proteins re sulted in B22 values well outside the crystallization slot.