CRYSTALLIZATION EXPERIMENTS WITH 2-ENOYL-COA HYDRATASE, USING AN AUTOMATED FAST-SCREENING CRYSTALLIZATION PROTOCOL

Authors
ZEELEN JP HILTUNEN JK CESKA TA WIERENGA RK
Citation
Jp. Zeelen et al., CRYSTALLIZATION EXPERIMENTS WITH 2-ENOYL-COA HYDRATASE, USING AN AUTOMATED FAST-SCREENING CRYSTALLIZATION PROTOCOL, Acta crystallographica. Section D, Biological crystallography, 50, 1994, pp. 443-447
Citations number
15
Categorie Soggetti
Crystallography,Biology,"Pharmacology & Pharmacy
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
50
Year of publication
1994
Part
4
Pages
443 - 447
Database
ISI
SICI code
0907-4449(1994)50:<443:CEW2HU>2.0.ZU;2-W
Abstract
A convenient method for screening crystallization conditions using an automated fast-screen protocol has been implemented and tested on an e noyl-CoA hydratase. The crystallization solutions for the initial scre ening and subsequent optimizations are prepared using a crystallizatio n robot. Enoyl-CoA hydratase (E.C. 4.2.1.17), purified from rat-liver mitochondria, is one of the enzymes from the beta-oxidation pathway of fatty-acid metabolism; it catalyzes the reversible hydration of 2-tra ns-enoyl-CoA's to L-3-hydroxyacyl-CoA's. Different crystal forms, diff racting to 3.0 angstrom, were obtained.