A GEL-MEDIATED FEEDING TECHNIQUE FOR PROTEIN CRYSTAL-GROWTH FROM HANGING DROPS

Authors
BERNARD Y DEGOY S LEFAUCHEUX F ROBERT MC
Citation
Y. Bernard et al., A GEL-MEDIATED FEEDING TECHNIQUE FOR PROTEIN CRYSTAL-GROWTH FROM HANGING DROPS, Acta crystallographica. Section D, Biological crystallography, 50, 1994, pp. 504-507
Citations number
10
Categorie Soggetti
Crystallography,Biology,"Pharmacology & Pharmacy
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
50
Year of publication
1994
Part
4
Pages
504 - 507
Database
ISI
SICI code
0907-4449(1994)50:<504:AGFTFP>2.0.ZU;2-S
Abstract
A procedure which allows an investigator to supply a crystal with fres h mother material without inducing significant growth defects is descr ibed. This technique requires that the crystal is grown in a gelled ha nging or sitting drop. An example concerning a model macromolecule, he n egg-white lysozyme, is given. Extension of this procedure to other m acromolecules is discussed.