Cf. Hildebolt et al., BITEWING-BASED ALVEOLAR BONE DENSITOMETRY - DIGITAL IMAGING RESOLUTION REQUIREMENTS, Dento-maxillo-facial radiology, 23(3), 1994, pp. 129-134
The resolution requirements were determined for detection of increment
al alveolar bone loss from digitized Ektaspeed radiographs. Ten clinic
al radiographs were examined with a calibrated optical microscope to m
easure the smallest feature of interest discernible for alveolar bone.
Images of trabeculae >100 mum in diameter could be identified, but sm
aller ones could not be resolved. The Nyquist Criterion requires sampl
ing with 500 mum (or smaller) pixels to measure such features. Numerou
s 25 mum Ag aggregates were present. Fifty mum resolution is a practic
al compromise between noise level and feature resolution. In another e
xample of 10 bitewings digitized at 8 bit grey scale, about half the a
vailable range was used for alveolar bone, resulting in uncertainty, o
ver a range of 2 optical density (OD) units, of about 1.42% at the ave
rage OD for alveolar bone (1.1). With the same radiographs digitized a
t 12 bit resolution, over 2000 of 4096 grey scales were used with a co
rresponding uncertainty of about 0.09%. Twelve-bit precision was also
able to resolve smaller increments in an aluminium stepwedge than was
8 bit precision. Twelve-bit grey scale and 50 mum spatial resolution a
re recommended for alveolar bone densitometry performed with Ektaspeed
radiographs.