ALPHA-CRYSTALLINS ARE INVOLVED IN SPECIFIC INTERACTIONS WITH THE MURINE GAMMA-D E/F-CRYSTALLIN-ENCODING GENE/

The promoter of the murine gamma E-crystallin (gamma E-cry) encoding g ene (gamma E-cry) was analyzed for specific interactions with lenticul ar proteins in a gel-retardation assay. A 21-bp fragment immediately d ownstream of the transcription initiation Site (DOTIS) is demonstrated to be responsible for specific interactions with lens extracts. The D O TIS-binding protein(s) accept only the sense DNA strand as target; a nti-sense or double-stranded DNA do not interact with these proteins. The DOTIS sequence element is highly conserved among the murine gamma D-, gamma E- and gamma F-cry and is present at comparable positions in the orthologous rat genes. Only a weak or even no protein-binding act ivity is observed if a few particular bases are changed, as in the rat gamma A-, gamma C- and gamma E-cry elements. DOTIS-binding proteins w ere found in commercially available bovine alpha-Cry preparations. The essential participation of alpha-Cry in the DNA-binding protein compl ex was confirmed using alpha-Cry-specific monoclonal antibody. The res ults reported here point to a novel function of alpha-Cry besides the structural properties in the lens.