Ag. Connolly et al., INTERPRETATION OF RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKER DATA FOR FINGERPRINTING SWEET-POTATO (IPOMOEA-BATATAS L) GENOTYPES, Theoretical and Applied Genetics, 88(3-4), 1994, pp. 332-336
In this paper we present a method for the generation of randomly ampli
fied polymorphic DNA (RAPD) markers for sweet potato. These were appli
ed to produce genetic fingerprints of six clonal cultivars and to esti
mate genetic distances between these cultivars. The level of polymorph
ism within the species was extremely high. From the 36-decamer random
primers used, 170 fragments were amplified, of which 132 (77.6%) were
polymorphic. Ten primers resulted in no detected amplification. Of the
remaining 26 primers for which amplification was achieved, only one d
id not reveal polymorphism. Six primers used alone enabled the discrim
ination of all six genotypes. Pattern analysis, which employed both a
classification and ordination method, enabled the grouping of cultivar
s and the identification of primers which gave greatest discrimination
among the cultivars.