DNA-FINGERPRINTING IN RICE USING OLIGONUCLEOTIDE PROBES SPECIFIC FOR SIMPLE REPETITIVE DNA-SEQUENCES

In this report we describe the use of five oligonucleotide probes, nam ely (GATA)(4), (GACA)(4), (GGAT)(4), (GAA)(6) and (CAC)(5), to reveal highly polymorphic DNA regions in rice. With each of the oligonucleoti de probes, the level of polymorphism was high enough to distinguish se veral rice genotypes. Moreover, individual plants of one cultivar show ed the same cultivar-specific DNA fingerprint. The multilocus fingerpr int patterns were somatically stable. Our study demonstrates that micr osatellite-derived DNA fingerprints are ideally suited for the identif ication of rice genotypes. As the majority of the probes detected a hi gh level of polymorphism, they can be very useful in monitoring and ai ding gene introgression from wild rice into cultivars.