J. Tan et al., ISOLATION, MAPPING, DNA-SEQUENCE AND RFLPS STUDIES OF RANDOM SINGLE-COPY DNA SEGMENTS ON HUMAN X-CHROMOSOME, Science in China. Series B, Chemistry, life sciences & earth sciences, 37(6), 1994, pp. 701-709
Using the total human/mouse DNA as the prober screening has been carri
ed out three times with in situ plaque hybridization to obtain the sin
gle-copy DNA sequence from the human X chromosome genomic library. The
effective rate of screening is 1.45%. DNAs from clones containing sin
gle-copy inserts have been analyzed by a panel of hybrid cells with or
without human X chromosome. Three segments , designated by DXFD52,73,
75, are mapped to the X chromosome. DXFD52 has been precisely localize
d on Xq12-q13 with in situ chromosomal hybridisation. DXFD52 has been
partially sequenced. The results indicate that DXFD52 is a new isolate
d single-copy segment on the X chromosome. Great progress in the RFLPs
study with DXFD52 has been achieved in the population of Chongqing, S
ichuan Province. The results show that the DXFD52 can be used to detec
t the RFLP with Hind III, Bgl II, and Hinf I. DXFD52 will be a potenti
al ''landmark'' for the construction of the complete linkage map of hu
man genome and the analysis of genomic sequence. And also, the results
provide the experience in the development of a useful DNA probe to be
applied to diagnosing X-linked disorders.