Cm. Changliu et Ge. Woloschak, EFFECT OF PASSAGE NUMBER ON CELLULAR-RESPONSE TO DNA-DAMAGING AGENTS - CELL-SURVIVAL AND GENE-EXPRESSION, Cancer letters, 113(1-2), 1997, pp. 77-86
The effect of different passage numbers on plating efficiency, doublin
g time, cell growth, and radiation sensitivity was assessed in Syrian
hamster embryo (SHE) cells. Changes in gene expression after UV or gam
ma-ray irradiation at different passage numbers were also examined. Th
e SHE cells were maintained in culture medium for up to 64 passages. C
ells were exposed to Co-60 gamma rays or 254-nm UV radiation. Differen
tial display of cDNAs and Northern blots were used for the study of ge
ne expression. With increasing passage number, SHE cells demonstrated
decreased doubling time, increased plating efficiency, and a decreased
yield in the number of cells per plate. Between passages 41 and 48 a
'crisis' period was evident during which time cell growth in high seru
m (20%) was no longer optimal, and serum concentrations were reduced (
to 10%) to maintain cell growth. Sensitivity to ionizing radiation was
no different between early- and intermediate-passage cells. However,
after UV exposure at low passages (passage 3), confluent cells were mo
re Sensitive to the killing effects of UV than were log-phase cells. A
t intermediate passages (passages 43, 48), confluent cells were slight
ly more radioresistant than were log-phase cells. By passage 64, howev
er, both confluent and log-phase cells showed similar patterns of UV s
ensitivity. Expression of gamma-actin, PCNA, and p53 transcripts did n
ot change following UV exposure. p53 mRNA was induced following gamma-
ray exposure of the intermediate (passage 45) epithelial cells. The ob
served differences in radiation sensitivity associated with increasing
passage number may be influenced by radiation-induced gene expression
. We are conducting experiments to identify these genes.