We present in this paper the use of a combined neurovascularized flap
of gracilis muscle and inguinal skin in the rat with the femoral vesse
ls and obturator nerve serving as the pedicles. The epigastric, saphen
ous, and muscular branch vessels arising from the femoral vessels were
preserved, and a portion of adductor magnus muscle was included in th
e flap to protect the delicate muscle vessels at their origins. The in
guinal skin and muscle flap both had independent blood supplies, thus
separate assessment of muscle and skin rejection was possible in the s
ingle transplanted ''packet.'' The muscle flap with the reconnected mo
tor nerve regained contractile ability upon nerve stimulation within 3
0 days after the iso-transplantation. The results suggest that the mod
ified gracilis myocutaneous flap provides an ideal model for transplan
tation research. (C) 1994 Wiley-Liss, Inc.