PREPARATION OF MONOCLONAL-ANTIBODIES FOR IMMUNOBLOTTING HUMAN GROWTH-HORMONE RECEPTOR AND GROWTH HORMONE-BINDING PROTEIN

Monoclonal anti-peptide antibodies against the extracellular domain of human growth hormone receptor (hGHR) were prepared. Four monoclonal a ntibodies (mAbs) reacted with an extracellular domain protein produced by genetic engineering. Among them, GHRP2-88 was the most reactive ag ainst hGHRs from human IM-9 cells. The lower limit of detection for im munoblotting using this mAb was about 200 pg hGHR. The GHRP2-88 antibo dy also reacted with deglycosylated hGHRs from tunicamycin-treated IM- 9 cells and with the growth hormone-binding protein (GH-BP) in human p lasma.