THE UGA4 UAS(NTR) SITE REQUIRED FOR GLN3-DEPENDENT TRANSCRIPTIONAL ACTIVATION ALSO MEDIATES DAL80-RESPONSIVE REGULATION AND DAL80 PROTEIN-BINDING IN SACCHAROMYCES-CEREVISIAE
Ts. Cunningham et al., THE UGA4 UAS(NTR) SITE REQUIRED FOR GLN3-DEPENDENT TRANSCRIPTIONAL ACTIVATION ALSO MEDIATES DAL80-RESPONSIVE REGULATION AND DAL80 PROTEIN-BINDING IN SACCHAROMYCES-CEREVISIAE, Journal of bacteriology, 176(15), 1994, pp. 4718-4725
Expression of the nitrogen catabolic genes in Saccharomyces cerevisiae
, including those of the gamma-aminobutyric acid (UGA) and allantoin (
DAL) pathways, is regulated positively by the GLN3 protein and negativ
ely by the DAL80 protein. The deduced sequences of the DAL80 and GLN3
proteins contain a zinc finger motif homologous to those shown to bind
GATA sequences. In addition, DAL80 protein has been directly shown to
bind to a pair of GATA-containing sequences (URS(GATA)) in vitro, and
a pair of GATA containing sequences (UAS(NTR)) is required for GLN3-d
ependent transcriptional activation in a heterologous expression vecto
r. We demonstrate here that the GATA-containing sites upstream of UGA4
required for optimal GLN3-dependent transcriptional activation also m
ediate DAL80 protein binding in vitro and DAL80-responsive regulation
in vivo