PARTICLE CONCENTRATION FLUORESCENCE IMMUNOASSAY CHEMOSENSITIVITY ASSAY FOR HUMAN EPITHELIAL OVARIAN-CANCER

We adapted particle concentration fluorescence immunoassay technology to develop a new chemosensitivity assay. Six human epithelial ovarian cancer cell lines were incubated with cisplatin for 24 hours. Cells we re then labeled with a fluorophore for 30 minutes. Removal of supernat ant, washing of tumor cells, and reading of epifluorescence was perfor med automatically. Percent cell lysis was calculated simultaneously by chromium release assay. The particle concentration fluorescence chemo sensitivity assay detected cell lysis in all six cell lines. The chemo sensitivity assay detected a larger percent lysis than the chromium re lease assay in each of the cell lines. The advantages of our chemosens itivity assay are plating efficacy, speed, efficiency, safety, and acc uracy.