CONSTITUENTS OF WERNERIA-CILIOLATA AND THEIR IN-VITRO ANTI-HIV ACTIVITY

The aerial part of Werneria ciliolata afforded a series of new diterpe nes: two ent-kaurane derivatives, a nor-kaurane, an ent-manoyloxide de rivative, a dimeric diterpene, as well as a rare diterpene. Their stru ctures were elucidated by spectroscopic methods, including the concert ed application of 1D NMR techniques (DEFT and NOEDS) and 2D NMR techni ques (H-1-H-1 COSY and HETCOR). In addition, four known kauranes, four coumarins and 6-hydroxytremetone were isolated. All isolated compound s from W. ciliolata and W. dactylophylla were tested in vitro for anti -viral activity against HIV-1, but only 6-hydroxytremetone showed a si gnificant anti-HIV-1 activity.