Wa. Hubert et al., RESPONSE OF CUTTHROAT TROUT EMBRYOS TO REDUCED INCUBATION TEMPERATURES AT DIFFERENT DEVELOPMENTAL STAGES, The Progressive fish-culturist, 56(3), 1994, pp. 185-187
We assessed the influence of developmental stage on the survival and g
rowth of embryos of cut-throat trout (Oncorhynchus clarki) fertilized
at 7-degrees-C and subsequently cooled to 5 or 3-degrees-C. Cooling fr
om 7-degrees-C took place after 2 h of water hardening, as well as 15,
25, and 35 d after fertilization. Mean survival to hatching was lowes
t (35%) among embryos cooled to 3-degrees-C immediately following wate
r hardening, but it was highest (94%) among embryos cooled to 3-degree
s-C following incubation at 7-degrees-C for 15 d. Mean survival among
embryos maintained at 7-degrees-C throughout incubation was 79%. Cooli
ng the embryos to 3-degrees-C after 15 d at 7-degrees-C prolonged the
time to hatching by 1 month and enhanced survival to hatching by 15% o
ver embryos maintained at 7-degrees-C.