EFFECT OF CHLORELLA, SPIRULINA AND CHLORO PHYLLIN ON FECAL EXCRETION OF POLYCHLORINATED DIBENZO-P-DIOXINS IN RATS

The present paper presents the liver distribution and fecal excretion of polychlorinated dibenzo-p-dioxins (PCDDs) congeners, such as 1,2,3, 7,8-P5CDD, 1,2,3,6,7,8-H6CDD, 1,2,3,1,8,9-H6CDD and 1,2,3,4,6,7,8-H7CD D, in male rats fed with Chlorella, Spirulina and chlorophyllin. The o bjective of this study is to evaluate the effect on PCDD excretion by the chlorophyllin foods. The rats were given some treatment diets cont aining 20% Chlorella. 20% Spirulina, 0.2, 2% chlorophyllin, 10% rice-b ran fiber or 0.2% chlorophyllin + 10% rice-bran fiber for 5 d. Then, t he animals were administered 4 g of each diet containing 0.5 ml of the causal rice-bran oil of Yusho that had occurred in the Southwest part of Japan in 1968 and kept on the same diet for another 5 d, The rice- bran oil contaminated with 1,2,3,7,8-P5CDD (6.86 ng/ml), 1,2,3,6,7,8-H 6CDD (31.4 ng/ml), 1,2,3,7,8,9-H6CDD (22.4 ng/ml) and 1,2,3,4,6,7,8-H7 CDD (121.7 ng/ml) was used for the animal experiments. PCDD congeners in the feces and liver were analyzed by high resolution gas chromatogr aphy-mass spectrometry. The fecal excretion of 1,2,3,7,8-P5CDD in the groups fed with Chlorella, Spirulina and 2% chlorophyllin were 7.4, 7. 1 and 11.0 times higher (p < 0.01). respectively, than that in the con trol group. Moreover, the fecal excretion of 1,2,3,6,7,8-H5CDD, 1,2,3, 7,8,9-H6CDD and 1,2,3,4,6,7,8-H7CDD in tile same groups were 4.4-5.3, 2.7-3.7 and 1.7-2.8 times higher, respectively, than that in the contr ol group. These findings suggest that administration of Chlorella, Spi rulina and chlorophyllin is useful as a new approach in the treatment of patients exposed to lipophilic xenobiotics.