An assay method is described for unlabeled, unpurified N(ative) and H(
eated) antigens of poliovirus. The method is based on competition betw
een the unlabeled antigen and a standard quantity of radiolabeled anti
gen, in the presence of a limiting amount of a N- or H-specific, monoc
lonal antibody. The immune complexes are removed by protein A-bearing,
fixed staphylococci. The method is free from cross-reaction between N
and H antigen, and has a detection limit of approximately 2 nM. It wa
s applied successfully to the quantitation of poliovirus antigen synth
esized by recombinant yeast expressing the viral proteins P1 and 3CD.