THE EFFECT OF HYDROLASES OF THE PREPARATI ON BIO-FEED-PLUS ON BARLEY UTILIZATION IN COMPLETE FEED MIXTURES FOR BROILER CHICKEN

Applications of enzymic preparations in feed rations for farm animals require the knowledge of their formulation, characteristics and behavi or in the presence of enzymes active in the digestive tract of the ani mal. Therefore it was necessary to determine the chemical composition and efficacy of the preparation (Tab. III). Optimum conditions of effi cacy of this enzymic preparation are at pH = 5 and at a temperature of 50-degrees-C. It can be supposed on the basis of the knowledge of opt imum conditions for cellulase activity contained in the preparation in which animals and in which part of their digestive tract the enzymic preparation will be effective. If enzymic preparations are really to b e utilized effectively in the nutrition of both monogastric animals an d ruminants, another factor should be taken into consideration, which is the effect of digestive juices produced in the parts of the gastroi ntestinal tract. Hence we investigated in an in-vitro trial the effect of pepsin in the HCl and trypsin medium on the cellulolytic activity of the enzymic preparation Bio Feed Plus. The obtained results (Tab. I Va) show that pepsin in HCl medium of pH = 2.1 considerably suppressed the cellulolytic activity of the preparation. In the presence of tryp sin at pH = 7.0 the cellulase activity was reduced only partly. These data indicate the importance of microgranulation treatment of the prep aration, or of coating microgranules with a protective layer. In a fee ding comparative trial with broilers, the effect of hydrolytic enzymes of this preparation was followed with respect to the growth and intak e of BR 1 and BR 2 mixtures while barley was used. Tab. II shows the e ffect of various concentrations of corn and barley in BR 1 and BR 2 mi xtures and the effect of the enzymic preparation Bio Feed on chicken w eight while Tab. V indicates interactions of the effect of various com and barley concentrations and applications of the enzymic preparation Bio Feed Plus in BR 1 and BR 2 mixtures on chicken weight. Dietary ba rley in BR 1 and BR 2 mixtures reduced the chicken weight highly signi ficantly (P < 0.01) in all the periods of observation. The terminal we ight of chickens which received barley instead of com was by 7.14% low er than in the control. Bio Feed Plus influenced the chicken weight hi ghly significantly positively in all the periods of investigation. At 21 days of age the weight of chickens which received dietary Bio Feed Plus was by 9.82% higher than in the control. at 49 days of age it was higher by 4.03% against the control. A study of interactions of the e nzymic preparation Bio Feed Plus and various amounts of dietary barley confirmed its positive effect on chicken growth. The weight of chicke n fed BR 1 and BR 2 mixtures with various amounts of barley when the p reparation Bio Feed Plus was administered at the same time was highly significantly higher than in the chickens fed mixtures with dietary ba rley but without the preparation. The weight of chickens which receive d mixtures with barley an Bio Feed Plus preparation was only by 1.5 to 2% lower than in the groups fed mixtures with the full amount of com. Tab. VI shows the values of feed intake per 1 kg weight gain and anal ysis of the different factors, Tab. VII indicates interactions of vari ous amounts of com and barley in the mixtures while the preparation Bi o Feed Plus was included in the feed mixes. The use of barley in BR 1 and BR 2 feed mixtures increased the total feed intake per 1 kg weight gain. Bio Feed Plus did not significantly influence the total feed in take per 1 kg weight gain. As shown by the results of the feeding comp arative trial, the use of barley in complete feed mixtures has negativ e effects on the performance figures in broiler chickens. The enzymic preparations Bio Feed Plus largely eliminated this effect in the param eter growth as it stimulated growth. Its effect on the feed intake per 1 kg weight gain was negligible.