THE EFFECT OF ADMINISTRATION OF BACILLUS CIP-5832 ON SOME PHYSIOLOGICAL-PARAMETERS AND PERFORMANCE OF PIGLETS AFTER WEANING

In two model and one feeding comparative trials with piglets after wea ning we investigated the effect of administration of 1 x 10(6) bacteri a of Bacillus C.I.P. 5832 per 1 g of COS feed mix on live weight, weig ht gain, feed intake per 1 kg weight gain, metabolic reaction in the c hyme of the small intestine and nutrient digestibility. The bacteria B acillus C.I.P. 5832, continually added to COS feed mixes, had positive effects on the weight gain of piglets (by 7.31 to 25.97 % higher agai nst the control at P < 0.1 to P < 0.05) and feed intake in kg per 1 kg weight gain (by 8.85 % to 8.89 % less than in the control). As indica ted by the results of all three trials with piglets after weaning, the probiotic preparation PACIFLOR had expressly positive effects on both the weight pin and the feed intake per 1 kg weight gain. Hence it is apparent that the bacteria Bacillus C.I.P. 5832, which are, an active ingredient of this preparation, positively influence the overall compo sition of the intestinal microflora and their action can eliminate the microbial shock caused by transition of sucking piglets to the intake of complete feed mixtures. The highly positive effect of these bacter ia was found with respect to die activity of alkaline and acid proteas es in the chyme of the small intestine, and consequently to the digest ibility of crude protein from ingested feed. The activity of acid prot eases in the chyme of the small intestine was on average 22.5 % higher while the activity of alkaline proteases was higher by 43 to 49 % (P < 0.1 to P < 0.05) in comparison with the control groups. The coeffici ent of nitrogen digestibility was by 3.57 % higher, coefficient of fat digestibility by 19.36 % higher and nitrogen utilization from ingeste d nitrogen by 4.02 % higher than in the control. The coefficient of di gestibility of nitrogen-free extract, coefficient of fiber digestibili ty and nitrogen utilization from digested nitrogen ranged within natur al variability. High production of hydrolytic enzymes, involving prote ase complex, is characteristic of some species of bacteria of the genu s Bacillus. This factor was investigated in two model trials. In both trials evenly, the activity of alkaline proteases in the chyme of the small intestine of piglets was found to be almost by 50 % higher in pi glets administered PACIFLOR in feed mix, if compared with the piglets of control groups. The increased activity of proteases was in agreemen t with the results of nutrient digestibility in trial 2, in which nitr ogen digestibility was by 3.5 % higher and utilization of ingested nit rogen was higher by 4 %.