IMPROVEMENT OF ANTIBODY SURFACE-DENSITY BY ORIENTATION OF REDUCED FRAGMENTS

Authors
AHLUWALIA A CARRA M DEROSSI D RISTORI C TUNDO P BOMBEN A
Citation
A. Ahluwalia et al., IMPROVEMENT OF ANTIBODY SURFACE-DENSITY BY ORIENTATION OF REDUCED FRAGMENTS, Thin solid films, 247(2), 1994, pp. 244-247
Citations number
12
Categorie Soggetti
Physics, Applied","Material Science","Physics, Condensed Matter
Journal title
Thin solid films → ACNP
ISSN journal
00406090
Volume
247
Issue
2
Year of publication
1994
Pages
244 - 247
Database
ISI
SICI code
0040-6090(1994)247:2<244:IOASBO>2.0.ZU;2-M
Abstract
A procedure for the orientation of antibody fragments at the gas-water interface is described and preliminary results are presented. The met hod exploits the presence of the reactive sulfhydryl group of a reduce d antibody fragment which can react with a floating monolayer. Surface pressure measurements show a strong interaction between the reduced f ragments and the monolayer. Deposited films are characterised by a flu orescent immunoassay and ellipsometry and the results indicate that th e antibody fragments are oriented with a high packaging density.