NOVEL METABOLITES OF WARFARIN PRODUCED BY BEAUVERIA-BASSIANA AND STREPTOMYCES-RIMOSUS - A NOVEL APPLICATION OF HPLC-NMR

1. Biotransformation of warfarin by the fungus Beauveria bassiana (ATC C 7159) yielded the first reported phase II warfarin metabolite, 3',4' -dihydroxywarfarin-3'-[4-methoxyglucoside], another previously unrepor ted metabolite, 3', 4'-dihydroxywarfarin and also 4'-hydroxywarfarin. 2. Biotransformation of warfarin by Streptomyces rimosus (NRRL 2234) y ielded the previously unreported metabolites, 12-hydroxywarfarin, 4'-h ydroxy-11-methoxywarfarin, and also 7-hydroxywarfarin and 4'-hydroxywa rfarin, which have not been previously reported as biotransformation p roducts from this organism. 3. Hplc-nmr has been used to identify biot ransformation products of warfarin by S. rimosus directly from the mic robial broth without prior isolation and purification.