MICROPATTERNING OF CULTURED-CELLS ON POLYSTYRENE SURFACE BY USING AN EXCIMER-LASER

Micropatterning of cultured cells on the surface of polystyrene was fo rmed using a 248 nm KrF excimer laser and less than 10 pulses. Adhesio n and proliferation of NG 108-15 neuroblastoma x glioma hybrid cells w ere good on the oxygen plasma treated polystyrene (O-PSt) surface, but poor on the PSt surface. The surfaces were characterized with a surfa ce profiler, scanning electron microscopy, contact angle microscopy, a nd x-ray photoelectron spectroscopy. When the laser ablation was carri ed out on a masked, O-PSt surface, a patterned surface architecture an d the micropattern of cultured cells were clearly obtained. A positive cell pattern was also achieved on the PSt surface by laser modificati on.