Cj. Hack et al., THE PRODUCTION OF PSEUDOMONAS-PUTIDA FOR THE HYDROXYLATION OF TOLUENETO ITS CIS-GLYCOL, Applied microbiology and biotechnology, 41(5), 1994, pp. 495-499
A constitutive blocked mutant (UV4) of Pseudomonas putida was grown in
a 2.5-1 fermenter on a mineral salts medium. Glucose was fed normally
over an 18-h period. Gluconate reached about 5 gl(-1) in the medium a
nd then fell to zero as it was utilised. The maximum toluene dioxygena
se specific activity (2 gg(-1) h(-1)) was obtained over the last 6 h o
f the fermentation when the pH was fully controlled. In fermentations
done at low dissolved O-2 tension (DOT) values there was an overall re
duction in the cellular enzyme level. When stored at 4 degrees C in ph
osphate buffer, pH 7.0, harvested bacteria lost half their activity in
about 90 h.