A SIMPLE AND INEXPENSIVE CHROMATOGRAPHIC METHOD FOR THE PURIFICATION OF GAMMA-GLOBULIN FROM HUMAN SERUM

Inexpensive cation- and anion-exchangers based on continuous polymer b eds were prepared directly in a plastic syringe. These beds are unique in the sense that they are synthesized by a very simple and cost-effe ctive procedure; for instance, no preparation of beads is required as in conventional methods. Highly purified gamma-globulin could be prepa red easily by step-wise elution accomplished by forcing buffers of inc reasing salt concentration through the column with the aid of the plun ger.