Cj. Digiorgio et al., QUANTITATIVE COMPUTER-ASSISTED IMAGE-ANALYSIS OF SUCTION BIOPSY IN PEDIATRIC GASTROESOPHAGEAL REFLUX, Pediatric pathology, 14(4), 1994, pp. 653-664
Gastroesophageal reflux is a disorder with well-characterized histopat
hological features in the adult, but the incidence and pathogenesis of
epithelial injury in children are poorly understood. Esophageal sucti
on biopsies from 80 infants exhibiting symptoms of reflux were studied
by computer-assisted image analysis and the results compared to routi
ne histological scoring. The histological features evaluated were the
number of intraepithelial lymphocytes and eosinophils, papillary heigh
t, interpapillary basal cell height, and a novel measure ''integrated
basal cell height.'' We quantitatively evaluated these histological cr
iteria by computer-assisted image analysis and compared these results
to four subjective grades of esophagitis: low, mild, moderate and high
. In this report we now describe this quantitative histopathologic met
hod for the evaluation of pediatric esophageal biopsies. Utilizing thi
s method we demonstrate that both inter- and intra-observer variabilit
y were sufficiently low to stratify the mucosal changes reliably into
at least four categories. The reliability of this objective analytic t
echnique will permit studies into the disease progression and regressi
on in pediatric reflux esophagitis.