En. Behrend et al., EFFECTS OF DIETARY-PROTEIN CONDITIONING ON GENTAMICIN PHARMACOKINETICS IN DOGS, Journal of veterinary pharmacology and therapeutics, 17(4), 1994, pp. 259-264
Eighteen, six-month-old male Beagles with normal renal function were r
andomly divided into three groups of 6. Each group was fed a diet that
was similar except for protein content (high = 26%, medium = 13% and
low = 9%, all on an as fed basis) throughout the experimental period.
After a 21 day dietary protein conditioning period (including a termin
al 2 day testing period), gentamicin was administered at a dosage of 1
0 mg/kg q. 8 h for 8 days. The first dose on days 1 and 7 was administ
ered i.v. and all others were given i.m. Pharmacokinetic parameters we
re determined using blood samples collected over an 8 h period followi
ng the i.v. dose on day 1. The elimination rate constant was calculate
d on days 1 and 7. The data best fit a two-compartment open model for
all dogs on day 1. The volume of distribution was higher and the clear
ance greater in the high protein group compared to the other two group
s. No difference was found in the rate of elimination between days 1 a
nd 7 for the high protein group; however, in the medium and low protei
n groups the rate of elimination decreased over the 7 days of treatmen
t. Therefore, high dietary protein prior to and during gent-amicin adm
inistration induced faster gentamicin clearance and a larger volume of
distribution and preserved the ability to eliminate gentamicin in dog
s with normal renal function.