NUCLEAR IMPORT OF MICROINJECTED INFLUENZA-VIRUS RIBONUCLEOPROTEINS

Influenza virus ribonucleoproteins (vRNPs) devoid of the matrix protei n (M1) were isolated and introduced into the cytoplasm of CHO and MDCK cells by microinjection. The injected vRNPs were found to be imported into the nucleus, and the RNA was transcribed. Their uptake into the nucleus was ATP-dependent, inhibited by antibodies to the nuclear pore complex, unaffected by the prior acidification of the vRNPs, and not inhibited by amantadine. The results showed that for productive infect ion, all the early stages of the viral entry pathway (receptor interac tion, endocytosis, acid exposure, and membrane fusion) can be bypassed . Once the vRNPs are stripped of M1 and separated from each other, the y are competent for import into the nucleus by constitutive cellular p rocesses. Second, the results showed that while the amantadine block f or incoming virus is manifested at the level of nuclear entry of the v RNPs, the actual import event per se is not affected. The results are consistent with a recent hypothesis that amantadine inhibits a step ne eded to prime the core for uncoating, which takes place before the vir us has reached the cytosol. (C) 1994 Academic Press, Inc.