HAIRPIN LOOP STRUCTURE AT THE TERMINI OF THE CHLORELLA VIRUS PBCV-1 GENOME

The termini of the chlorella virus PBCV-1 330-kb dsDNA genome consist of 35-nucleotide-long, incompletely base-paired, covalently closed hai rpin loops that exist in one of two forms. The two forms are complemen tary when the 35 nucleotide sequences are inverted with respect to one another (flip and flop). Each hairpin loop structure is followed by a n identical 2221-bp inverted repeat sequence after which the DNA seque nce diverges. The strategy for cloning the PBCV-1 DNA hairpin ends may be useful for cloning other hairpin termini. (C) 1994 Academic Press, Inc.