Dp. Heller et Gp. Raaphorst, INHIBITION OF POTENTIALLY LETHAL DAMAGE RECOVERY BY ALTERED PH, GLUCOSE-UTILIZATION AND PROLIFERATION IN PLATEAU GROWTH-PHASE HUMAN GLIOMA-CELLS, International journal of radiation biology, 66(1), 1994, pp. 41-47
Citations number
29
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging","Nuclear Sciences & Tecnology
Recovery from potentially lethal damage (PLD) has been measured in pla
teau growth phase human glioblastoma cells (U-87MG) under four postirr
adiation medium conditions. Recovery was maximal in depleted medium at
an acidic pH, conditions which inhibit cellular proliferation. Compar
ed with this control, PLD recovery (PLDR) was increasingly inhibited b
y alkalization of the existing medium (to pH 7.4), exchanging the old
medium with fresh medium-pH acidified (to pH 6.8), and exchanging the
old medium with fresh medium-pH unaltered (pH 7.4), respectively. Thes
e three medium adjustments were made at the time of irradiation. Incre
ased glucose utilization (glycolysis) was detected postirradiation in
all three cases, while increased proliferation was detected only when
fresh medium was exchanged for old medium. Thus inhibition of PLDR has
been correlated with increased glycolysis and increased proliferation
during the recovery period. When acting together, these two processes
provided almost complete inhibition. This study was revealed that the
degree of inhibition may be related to the amount of glycolysis and/o
r proliferation occurring during the recovery period. Examining, in vi
tro, the range of PLDR achieved by postirradiation manipulation of med
ium pH may provide some indication of the range in PLDR that may be ex
pected in vivo. Our study demonstrates that the effect of pH on glycol
ysis and proliferation may be important when determining the ability o
f a particular cell type to recover from PLD.