SEQUENCE-ANALYSIS OF A 31 KB DNA FRAGMENT FROM THE RIGHT ARM OF SACCHAROMYCES-CEREVISIAE CHROMOSOME-II

The nucleotide sequence of a 31 352 bp fragment from chromosome II of Saccharomyces cerevisiae has been determined and analysed. The fragmen t originates from the right arm of chromosome II, located between the GAL7,10,1 and the PHO3,5 loci, at a distance of about 130 kb from the centromere. The sequence contains a tRNA tandem repeat and 17 open rea ding frames (ORFs) larger than 100 amino acids. One of them extends in to adjacent DNA and is incomplete. The two tRNA genes, coding for a tR NA(asp) and a tRNA(arg), and three of the ORFs, had been sequenced pre viously, i.e. HSP26, SEC18, and UBC4. Four other ORFs showed similarit y with yeast genes; amino acid transporter genes, the RAD54, SNF2 and STH1 family, the SPS2 gene and the bromodomain of SPT7, respectively. Two showed homology with sequences from other organisms, i.e. with a P lasmodium falciparum gene encoding a surface antigen and with a gene f rom Saimirine herpes virus respectively. Three ORFs, YBR0726, YBR0735 and YBR0740 are completely contained in YBR0727, YBR0734 and YBR0739 r espectively, and thus probably do not represent real genes. Two ORFs, YBR0727 and YBR0745 most likely contain an intron. The sequences have been deposited in the EMBL data library under Accession Number X76294.