ALKALOIDS FROM HIPPEASTRUM HYBRIDS

The alkaloid 11-hydroxyvittatine has been isolated from bulbs of Hippe astrum (hybrids) for the first time. Until now it was only found in pl ants belonging to the genus Sternbergia [1]. The bulbs additionally co ntain the known alkaloids vittatine, montanine, pancracine, lycorine, tazettine, haemanthamine and hippeastrine. By comparison with the lite rature it turned out that the structure elucidation based on NMR signa ls' assignment is still incomplete for some cases. The alkaloids vitta tine, 11-hydroxyvittatine and montanine were investigated by means of one and two dimensional H-1 and C-13 NMR. The overlapping signals in t he H-1 NMR spectra of this alkaloids required 2D-techniques like HOHAH A (homonuclear Hartmann-Hahn spectroscopy), ROESY (rotating frame Over hauser effect spectroscopy) as well as H-1, C-13 HMBC (heteronuclear m ultiple bond correlation) for the signals' assignment. The application of this techniques is described to point out their general capability in structure elucidation of alkaloids.