THE SEPARATION OF BIOLOGICALLY-ACTIVE DIASTEREOMERS OF ZWITTERIONIC BETA-LACTAMS BY ANION-EXCHANGE CHROMATOGRAPHY

Authors
GOODEN WE DU Y AHLUWALIA R DAY RA
Citation
We. Gooden et al., THE SEPARATION OF BIOLOGICALLY-ACTIVE DIASTEREOMERS OF ZWITTERIONIC BETA-LACTAMS BY ANION-EXCHANGE CHROMATOGRAPHY, Analytical letters, 27(11), 1994, pp. 2153-2169
Citations number
17
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical letters → ACNP
ISSN journal
00032719
Volume
27
Issue
11
Year of publication
1994
Pages
2153 - 2169
Database
ISI
SICI code
0003-2719(1994)27:11<2153:TSOBDO>2.0.ZU;2-G
Abstract
Separation of diastereomeric zwitterionic beta-lactams was effected on an anion-exchange column. An aqueous pH gradient from neutrality to p H 1 was generated using hydrochloric acid with the resin in the chlori de form. Isocratic separations were done with 0.1 N acetic acid with t he resin in the acetate form. The separated products were characterize d by infrared spectroscopy, mass spectrometry, beta-lactamase inhibito ry activity and antibiotic activity.