STUDY ON THE FLUORESCENCE SYSTEM OF TB-ATP-PHEN FOR THE DETERMINATIONOF ATP

Authors
YANG JH ZHOU GJ JIE NQ GAO ZQ ZOU HB YOU CP HAN Y
Citation
Jh. Yang et al., STUDY ON THE FLUORESCENCE SYSTEM OF TB-ATP-PHEN FOR THE DETERMINATIONOF ATP, Analytical letters, 27(11), 1994, pp. 2181-2193
Citations number
6
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical letters → ACNP
ISSN journal
00032719
Volume
27
Issue
11
Year of publication
1994
Pages
2181 - 2193
Database
ISI
SICI code
0003-2719(1994)27:11<2181:SOTFSO>2.0.ZU;2-R
Abstract
It was found that ATP could form a ternary complex with Tb3+ and Phen, which can emit the characteristic fluorescence of Tb3+. The experimen t showed that the fluorescence intensity of the system was maxmium in the following conditions: pH of the solution is 7.2, the concentration s of Tb3+ and phenanthroline (Phen) are 2.0X10(-5)mol.L(-1) and 2,0X10 (-4)mol.L(-1), respectively. At the optimum conditions, a linear relat ionship is obtained between the fluorescence intensity and ATP concent ration in the range of 1.0x10(-6)-1.0.X10(-5)mol.L(-1). Traces of ATP in drugs (ATP disodium salt tablets) can be determined using the stand ard addition method. The luminescence mechanism of the system was disc ussed.