THE STRUCTURAL INVESTIGATIONS OF THE MITO CHONDRIAL H-ATPASE()

Authors
GRINKEVICH VA BELOGRUDOV GI ZAYTSEV VG PAVLOV PF ILJINA EF NAZIMOV IV
Citation
Va. Grinkevich et al., THE STRUCTURAL INVESTIGATIONS OF THE MITO CHONDRIAL H-ATPASE(), Biologiceskie membrany, 11(3), 1994, pp. 310-323
Citations number
56
Categorie Soggetti
Cytology & Histology
Journal title
Biologiceskie membrany → ACNP
ISSN journal
02334755
Volume
11
Issue
3
Year of publication
1994
Pages
310 - 323
Database
ISI
SICI code
0233-4755(1994)11:3<310:TSIOTM>2.0.ZU;2-I
Abstract
The results on the investigations of the topography of the OSCP from b ovine heart mitochondria are shortly reviewed. Hydrolysis of OSCP from pig heart mitochondria by proteinase from Staphylococcus aureus and h ydrolysis of the preliminary citraconylated OSCP by trypsin were folow ed by isolation of the individual peptides by means of HPLC. Structura l analysis of these peptides allowed us to reconstruct the complete am ino acid sequence of this protein. Comparative structural analysis rev ealed existence of the close homology (93.2%) between the pig OSCP and the bovine OSCP. Some amino acid substitutions are localized close to linear antigenic determinants of these proteins, or even within the a mino acid sequence or some determinants.