CLONING OF CDNA-ENCODING S26 HUMAN RIBOSO MAL-PROTEIN AND ITS SEQUENCE-ANALYSIS

The polymerase chain reaction technique was employed to isolate cDNA e ncoding S26 human ribosomal protein. Based on the known sequence of ra t S26 ribosomal protein we have designed primers and amplified the cor responding sequence of human cDNA from total placenta cDNA. The fragme nt of S26 cDNA was cloned in a plasmid vector and sequenced by the San ger method. Extremely high homology (87,7%) between coding regions of S26 mRNAs in rat liver and human placenta was revealed. The only amino acid substitution (Ser --> Val) at position 38 was observed. Results of blot hybridization with partially digested human genomic DNA sugges t the presence of more than 6 copies of the S26 gene.