MECHANISMS OF INTERACTIONS OF SOME PHENYL BENZOIMIDAZOLE AND PHENYLINDOLE DYES WITH DNA

Interactions of several phenylbenzoimidazole and phenylindole dyes wit h DNA were investigated by means of absorption and fluorescent spectra in aqueous solutions. Relative contributions of ionic and hydrogen bo nds in to these interactions were determined. Spectral properties of t he pure dyes in isopropanol were studied to evaluate the influence of microenvironmental factors on the emission of potentially active fluor ochromes. Several parameters of the dyes' binding with nucleic acids w as calculated basing on spectral data. Relations among some binding ch aracteristics and spectral parameters of the dyes are presented. A ser ies of terminal electronic donor-acceptor substituentes for the direct ed synthesis of new fluorochromes for DNA are suggested and requiremen ts for their application for several classes of compounds are formulat ed.