Ha. Zenkevich et al., CHARACTERIZATION OF CARBOHYDRATE CHAINS O F 2 FORMS OF HUNCHBACK SALMON GONADOTROPIC HORMONE HAVING DIFFERENT AFFINITY TO CONCANAVALIN-A, Bioorganiceskaa himia, 20(6), 1994, pp. 682-690
A comparative analysis of carbohydrate chains of two forms of hunchbac
k salmon gonadotropin that bind or not with ConA-Sepharose was carried
out. It was found that unbound, <<protein>>, ConA--form contains thre
e times less carbohydrates and has slightly different amino acid compo
sition as compared to the bound, normally glycosylated ConA+-form. HPL
C fractionation showed the oligosaccharides released from both hormone
forms to be mainly sialylated. The major oligosaccharides identified
in ConA-form are biantennary, fucosylated (approximately 20%) or nonfu
cosylated; minor bissected chains are also present. In ConA--form the
same oligosaccharides and also oligosaccharides with a higher degree o
f branching (tri- and tetraantennary) were detected. These two hormone
forms isolated from pituitary glands of male or female fish differed
neither in the type of oligosaccharides nor in their ratio. Essential
structural differences in carbohydrate chains of hunchback salmon and
sturgeon gonadotropins were distinctly demonstrated.