A. Concetti et al., IMMUNORECOGNITION OF RING SKELETON OF TAXANES BY CHICKEN EGG-YOLK ANTIBODIES, Biological chemistry Hoppe-Seyler, 375(6), 1994, pp. 419-423
Anti-10 deacetylbaccatin III (DAB) antibodies (IgY) were elicited in h
ens immunized with a succinyl-DAB/BSA conjugate and extracted from egg
yolk. As shown by indirect competitive inhibition enzyme immunoassay
(CIEIA), the addition of free-DAB competitively inhibited the binding
of affinity purified anti-DAB IgY to DAB/BSA solid phase conjugated an
tigen. The assay enabled the detection of DAB in concentrations as low
as 7.5 ng/ml (13.7 nM DAB), whereas anti-DAB IgY did not react with t
axol even at a concentration a thousand times higher. The structural r
equirements of the diterpenoid nucleus for binding to IgY were conside
red on the basis of the levels of cross-reaction found with 10 authent
ic taxanes. The results indicate that anti-DAB IgY represents the firs
t high affinity antibody produced capable of recognizing the ring skel
eton of taxol precursors.